Antibody affinity determination and quantification
Label-free analysis for affinity of analyte-ligand binding (KD, ka and kd) and quantitation in real-time.
Analytes can be measured in crude, unpurified mixtures such as cell lysates or hybridoma supernatants. Bio-layer Interferometry (BLI) technology analyzes quantitative and qualitative intermolecular interaction with the advantages of:
- High sensitivity
- High throughput
- Low sample volume
Biocult proudly offers you affinity measurements using the Octet® R8 system from Sartorius for reliable analysis of:
• Kinetic analysis
• Quantification analysis
• Antibody pairing for immunoassay development
The Octet® platform based on BLI technology analysis service includes a set of:
• General biosensors
• Assay kit to support the evaluation of biomolecular interactions in microplates
We are dedicated to delivering top-quality Bio-layer Interferometry technology services to you. Allow us to assist you, take away concerns about your research investments and meeting deadlines with confidence. Biocult is your trusted partner for Biolayer Interferometry technology, a pivotal analysis that drives your projects towards success by focusing on specified intended interactions. Together, we can create greatness.
Biomolecular interactions studies for you
Label-free analysis for the determination of affinity analyte-ligand binding antibody pairing and antibody/protein quantitation can be important
The Octet® system has several advantages compared to other techniques that are label-free such as Surface Plasmon Resonance (SPR). The benefits are:
- A wide range of ligand biosensor surfaces that allow for immobilization of antibodies, recombinant proteins, DNA, RNA and virus-like particles
- Fast results, protein/antibody quantitation in as little as two minutes, and parallel processing of up to 96 samples
We are not only experienced in determining the specifications of your antibodies/proteins but we also have the knowledge and technology to produce antibodies and ready to use assays.
Are you in search of a partner to collaborate on the development of an assay utilizing your antibodies? This is just one of the numerous services we offer, following the analysis and production of your antibodies/proteins. Please share your requirements with us so that we can tailor our assistance to your specific needs.
- Consultancy for your production
- Perform experiments to determine the specificity of your antibody
- Test the suitability of your antibody in several techniques, like: BLI, flow cytometry, western blot or ELISA
- Translation of your antibody into an assay
- Upscale your antibody production up to gram scale
Need some help before starting and professional advice? Consultancy before starting your project is also possible.
You minimize expenses and maximize reaching your objectives
Choose Biocult and make your bio-layer interferometry analysis more effective and efficient!
At Biocult we believe that the best results are obtained if we think along with your needs. We strive to do this in a smart and practical way, by always searching for the best custom solutions to get the results or products you are looking for. With more than 25 years of experience and knowledge we can translate your thoughts to products, research objectives and more.
- We think along in a smart and practical way, always searching custom solutions and looking for the best results
- We work in a personal way with knowledge of antibody production and creation process
- We act together and are committed from start to finish
- We believe in dedicated contacts and short and transparent communication
Do not hesitate to get in contact and share your thoughts with us about your needs, objectives or wishes for BLI. You can contact us via e-mail or give us a call to discuss all options.