Antibody affinity determination and quantification

Octet^® BLI systems enable label-free analysis for affinity of analyte-ligand binding (KD, ka and kd) and quantitation in real-time.

Analytes can be measured in crude, unpurified mixtures such as cell lysates or hybridoma supernatants. Biolayer Interferometry (BLI) technology analyzes quantitative and qualitative intermolecular interaction with the advantages of:

• High sensitivity
• High throughput
• Low sample volume

We are committed to provide you our BLI technology service with the best quality. Biocult proudly offers you affinity measurments using the Octet^® R8 system from Sartorius for reliable analysis of:

• Kinetic analysis
• Quantification analysis
• Antibody pairing for immunoassay development

The Octet^® platform based on bio-layer interferometry (BLI) technology analysis service includes a set of:

• General biosensors

• Assay kit to support the evaluation of biomolecular interactions in microplates

Let us help you and stop worrying about your research investments and deadlines. We believe Biocult is the right company to help you with Biolayer Interferometry technology. A key essential analysis which drives your projects to success based on specified intended interactions. Together, we can create greatness.

We understand label-free analysis for the determination of affinity analyte-ligand binding antibody pairing and antibody/protein quantitation can be important.

The Octet^® system has several advantages compared with other techniques which are label-free such as Surface Plasmon Resonance (SPR).  The benefits are:

• A wide range of ligand biosensor surfaces that allow for immobilization of antibodies, recombinant proteins, DNA, RNA and virus-like particles
• Fast results, protein/antibody quantitation in as little as two minutes, and parallel processing of up to 96 samples 

Get in contact with Biocult and share your thoughts with us. We are not only experienced in determining the specifications of your antibodies/proteins but we also have the knowledge and technology to produce antibodies ready to use assays and immuno assay development.

Perhaps are you looking for a partner to develop an assay with your antibodies? This is one of the many services we can do for you after your antibodies/proteins analysis and production. Please let us know how can we assist you?

• Consultancy for your production
• Perform experiments to determine the specificity of your antibody
• Test the suitability of your antibody in several techniques, like: BLI, flow cytometry, western blot or ELISA
• Translation of your antibody into an assay
• Upscale your antibody production up to gram scale

Need some help before starting and professional advice? Consultancy before starting your project is also possible.

Choose Biocult and make your bio-layer interferometry analysis more effective and efficient!

At Biocult we believe that the best results are obtained if we think along with your needs. We strive to do this in a smart and practical way, by always searching for the best custom solutions to get the results or products you are looking for. With more than 25 years of experience and knowledge we can translate your thoughts to products, research objectives and more.

• We think along in a smart and practical way, always searching custom solutions and looking for the best results
• We work in a personal way with knowledge of antibody production and creation process
• We act together and are committed from start to finish
• We believe in dedicated contacts and short and transparent communication

Do not hesitate to get in contact and share your thoughts with us about your needs, objectives or wishes for BLI. You can contact us via e-mail or give us a call to discuss all options.