Validation of Anitbodies

Together, we create greatness

Our Antibody Validation Services are designed to help you confidently move forward, knowing that your antibodies are rigorously tested and validated in the application of your choice: Flow Cytometry, Western blot, ELISA, and/or IHC. 

  • Antibody Specificity Testing: We verify that the antibody binds specifically to the target of interest. 
  • Antibody Sensitivity Testing: We determine the lower limit of detection for your antibody. 
  • Cross-reactivity Analysis: Assess potential cross-reactivity with related proteins. 
  • Antibody Characterization: Comprehensive antibody characterization, including isotype determination and purity assessment. 

At Hycult Biotech we use the Bio-layer interferometry technology Octet (Sartorius) as a versatile and powerful tool for assessing the binding kinetics, specificity, and sensitivity  of antibody-antigen interactions. 

The Octet R8 contains eight spectrophotometers, allowing up to eight samples to be read simultaneously. This instrument includes glass biosensors, the size of a 10 µL pipette tip, coated with a biological component. Depending on the application and desired outcome, the most suitable biosensor can be selected. The Octet is based on Biolayer Interferometry (BLI) which is a dip-and-read system in which the biosensors are moved between different positions in a 96-well plate. BLI is used for label-free, real-time analysis of biomolecular interactions. During measurement, bio-layer interferometry (BLI) detects the interactions occurring on the biosensors. It is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces.  

The Octet R8 system is equipped with eight spectrophotometers, enabling simultaneous analysis of up to eight samples. The instrument utilizes glass biosensors each approximately the size of a 10 µL pipette tip coated with a specific biological component. Depending on the application and experimental goals, different biosensor types can be selected for optimal performance. 

The Octet operates on the principle of Biolayer Interferometry (BLI), a label-free, real-time analytical technique used to study biomolecular interactions. In this dip-and-read system, biosensors are sequentially transferred between wells of a 96-well plate. BLI measures changes in the interference pattern of white light reflected from two surfaces within the biosensor, allowing precise detection and quantification of binding events occurring on its surface. 

During an experiment, the biosensors of the Octet are illuminated with white light. The reflection of this light from the biosensors immersed in buffer serves as a reference signal (blue). When molecules such as immobilized proteins bind to the biosensor surface, the optical properties change, causing a measurable shift in the interference pattern (orange). This shift, represented as a change in wavelength (Δλ), is directly proportional to the number of bound molecules. Because Biolayer Interferometry (BLI) measures these changes in real time, it enables precise determination of key kinetic parameters, including association, dissociation, affinity, and concentration. The resulting data are displayed as a binding curve, plotting the wavelength shift against time. 

Biosensors are exposed to white light. Reflection of the light of a biosensor in buffer serves as reference (blue). When molecules get immobilized to the sensor,
the reflection changes and a shift of the interference pattern occurs (orange). This shift can be translated to a binding curve on the OCTET

Looking to analyze your proteins or antibodies? Get expert support from our team. Choose Biocult and make your work easier. We’re here to help when your company faces capacity limits or expertise shortages. At Biocult, we combine personal commitment with deep technical know-how to deliver tailor-made, high-quality results. Our practical, solution-driven approach ensures efficiency and excellence from start to finish. With open communication, trusted collaboration, and full EN ISO 13485:2016 compliance, Biocult is your partner for reliable contract manufacturing and contract research empowering your success, every step of the way. 

Our Service

Confidently validate your antibodies with Biocult’s Octet R8 system, providing accurate, real-time binding analysis for specificity, sensitivity, and cross-reactivity.

Curious what we can do for you? Contact us and let’s create greatness together!

Contact us
Biocult