Advances in Cell Culture for Clinic and Pharmaceutical Industry

The culture and expansion of cells is one of the basic methodologies in immunological, biomedical and biotechnological sciences. The emerge of a greater interest in biotechnology and its products for diagnostic and therapeutic approaches in the last 10-20 years has also stimulated the improvement and extension of methodology for large scale handling and expansion of animal and human cells, stem cells and tissues as well as other cells.

Implementation of bioreactors, needed for medium scale and large (industrial) scale in vitro production and purification of cellular proteins, enables the production of larger amounts of well-defined cell derived proteins such as monoclonal antibodies for diagnostic and clinical use.

Optimisation of productivity requires a better understanding of cell physiology, apoptosis, growth-factors and culture media composition, especially in high cell density cultures: hydrodynamics, oxygen and/or shear stress and differences between different types of bioreactors. Information on glycosylation patterns is also important since these can have great impact especially on in vivo efficacy in therapeutic schemes. Also here the differences in type of bioreactor may be important.

This course will focus on theoretical and practical, operational aspects, implementation of methodological and technical aspects, as well as on regulatory requirements and facilities for large scale expansion of cells (fermentation) and purification (down stream processing) of cell products for medical use. Hands-on as well as video demonstrations and excursions will be included. The pros and contras of the various methods will be discussed. In addition an update will be given of recent developments and (clinical) applications of,  humanized therapeutic antibodies, recombinant proteins, gene therapy and stem cell therapy.

Who Should Attend

Participation to the course is aimed at postgraduate junior and senior scientist, (M.D., Ph.D. or Engineers in live sciences such as cell biology, immunology, medicine and biotechnology, involved in development and/or up-scaling/fermentation or purification of biotechnological products.

R&D and production managers with a multidiscipline interest who wish to broaden their scope and/or to update their knowledge on recent advances and prospects will also benefit from participation to this course